HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: db07-1615v1 57/7/1790    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Berglund, E. D. Articles by Wasserman, D. H. Search for Related Content PubMed PubMed Citation Articles by Berglund, E. D. Articles by Wasserman, D. H. Social Bookmarking                What's this?

Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains

¹ Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee
² Vanderbilt University–NIH Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, Tennessee
³ Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, Vanderbilt University School of Medicine, Nashville, Tennessee
⁴ Departments of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina
⁵ VA Tennessee Valley Healthcare System, Nashville, Tennessee

Corresponding author: Eric Berglund, eric.d.berglund{at}vanderbilt.edu

OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains.

RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (8.5 mmol/l) and -hypoglycemic (3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets.

RESULTS—Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets.

CONCLUSIONS—Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?